Development of Electrochemical Biosensor for
Detection of Cr3+, Fe2+ and Pb2+ in Water Matrix
Buba Mohammed
Department of Science Laboratory Technology Federal Polytechnic, Mubi. Adamawa State Nigeria
ABSTRACT
The aim of this research work is to develop an electrochemical platform for detection of Cr3+, Fe2+ and Pb2+ in Water Matrix using Velvet beans urease enzymes immobilized on a transducer (glassy carbon electrode) as bioactive membrane. Biochemical characterization of the urease enzymes was carried out using UV-visible spectrophotometer, while the electrochemical characterization, optimization of bioactive membrane and analysis were carried out on electrochemical work station (AUTO LAB). Validation studies was carried out with AAS as standard method. Results obtained for the overall work showed convincing evidence for biosensor development. Electrochemical studies of the immobilized bioactive membrane showed enhanced peak with GCE/ZnONPs/urease, the developed biosensor showcased excellent performance with 1 µl urease enzyme and 1.5 mg/l ZnONPs as bioactive membrane, the life time of the developed biosensor was estimated to be 7 days. Excellent reproducibility of six independently prepared bioactive platforms was observed with a relative standard deviation of 4.23%. Validation results showed good recoveries for all the metals tested with the developed biosensor and AAS. Therefore, the developed biosensor can be rated with high score and recommended for the analysis of the selected metals in water samples.
Keywords: Urease, Velvet beans, Biosensor, Heavy metals
CITE AS: Buba Mohammed (2026). Development of Electrochemical Biosensor for Detection of Cr3+, Fe2+ and Pb2+ in Water Matrix. INOSR APPLIED SCIENCES 14(1):18-29. https://doi.org/10.59298/INOSRAS/2026/14.1.1829000
TETFund Supported Publication